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mpex pts reagent  (GL Sciences)

 
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    GL Sciences mpex pts reagent
    Mpex Pts Reagent, supplied by GL Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mpex pts reagent/product/GL Sciences
    Average 90 stars, based on 1 article reviews
    mpex pts reagent - by Bioz Stars, 2026-04
    90/100 stars

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    mpex pts reagent 5010-21360  (GL Sciences)
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    GL Sciences mpex pts reagent 5010-21360
    (A) Schematic flow of shotgun proteomics analysis. Shotgun proteomics analyses were carried out from both the soluble and insoluble fractions after the treatment of the PSDL preparations with <t>MPEX</t> <t>PTS</t> reagent. (B) Bar graphs of PSDL samples prepared by the initial and new methods, which show the proportion of protein numbers identified in the MPEX PTS–soluble, insoluble, and both fractions, respectively. The graph shows a significant improvement in the protein solubility of the MPEX PTS in the new PSDL. The effect may be similar compared with the other initial PSDL preparation (1% OG-IS-11U), which also showed insolubility in SDS/mercaptoethanol. See for sample names of initial PSDL.
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    (A) Schematic flow of shotgun proteomics analysis. Shotgun proteomics analyses were carried out from both the soluble and insoluble fractions after the treatment of the PSDL preparations with MPEX PTS reagent. (B) Bar graphs of PSDL samples prepared by the initial and new methods, which show the proportion of protein numbers identified in the MPEX PTS–soluble, insoluble, and both fractions, respectively. The graph shows a significant improvement in the protein solubility of the MPEX PTS in the new PSDL. The effect may be similar compared with the other initial PSDL preparation (1% OG-IS-11U), which also showed insolubility in SDS/mercaptoethanol. See for sample names of initial PSDL.

    Journal: Life Science Alliance

    Article Title: Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices

    doi: 10.26508/lsa.202000945

    Figure Lengend Snippet: (A) Schematic flow of shotgun proteomics analysis. Shotgun proteomics analyses were carried out from both the soluble and insoluble fractions after the treatment of the PSDL preparations with MPEX PTS reagent. (B) Bar graphs of PSDL samples prepared by the initial and new methods, which show the proportion of protein numbers identified in the MPEX PTS–soluble, insoluble, and both fractions, respectively. The graph shows a significant improvement in the protein solubility of the MPEX PTS in the new PSDL. The effect may be similar compared with the other initial PSDL preparation (1% OG-IS-11U), which also showed insolubility in SDS/mercaptoethanol. See for sample names of initial PSDL.

    Article Snippet: MPEX PTS reagent , 5010-21360, 21361 , Not found , GL Sciences Inc..

    Techniques: Solubility

    Proteins in the three PSDL preparations: initial PSDL/1% OG-IS-11B , lean-type PSDL (1% OG, U), and enriched-type PSDL (0.75% OG, U), respectively, were recovered by the chloroform/methanol protocol, and solubilized with MPEX PTS reagent. Proteins in both MPEX PTS-solubilized and insoluble fractions were comprehensively identified by mass spectrometry using the shotgun method . Protein distribution in these samples was compared with Thermo Proteome Discoverer. (A, B) Comparison of total proteins (A) and MPEX PTS-insoluble components (B) among the three preparations are shown in the Venn diagram. (C) Grouping of the PSDL components into the minimum essential cytoskeleton (MEC) and non-MEC components. (B) MEC is the same as the gray area that contains 58 protein species in (B). The protein numbers in each area are indicated in parentheses. For protein components of MEC and non-MEC, see Tables S3 and S4.

    Journal: Life Science Alliance

    Article Title: Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices

    doi: 10.26508/lsa.202000945

    Figure Lengend Snippet: Proteins in the three PSDL preparations: initial PSDL/1% OG-IS-11B , lean-type PSDL (1% OG, U), and enriched-type PSDL (0.75% OG, U), respectively, were recovered by the chloroform/methanol protocol, and solubilized with MPEX PTS reagent. Proteins in both MPEX PTS-solubilized and insoluble fractions were comprehensively identified by mass spectrometry using the shotgun method . Protein distribution in these samples was compared with Thermo Proteome Discoverer. (A, B) Comparison of total proteins (A) and MPEX PTS-insoluble components (B) among the three preparations are shown in the Venn diagram. (C) Grouping of the PSDL components into the minimum essential cytoskeleton (MEC) and non-MEC components. (B) MEC is the same as the gray area that contains 58 protein species in (B). The protein numbers in each area are indicated in parentheses. For protein components of MEC and non-MEC, see Tables S3 and S4.

    Article Snippet: MPEX PTS reagent , 5010-21360, 21361 , Not found , GL Sciences Inc..

    Techniques: Mass Spectrometry, Comparison

    List of major chemicals used in this study.

    Journal: Life Science Alliance

    Article Title: Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices

    doi: 10.26508/lsa.202000945

    Figure Lengend Snippet: List of major chemicals used in this study.

    Article Snippet: MPEX PTS reagent , 5010-21360, 21361 , Not found , GL Sciences Inc..

    Techniques: Staining, Protease Inhibitor, Silver Staining